Next-generation disulfide stapling: reduction and functional re-bridging all in one
نویسندگان
چکیده
منابع مشابه
Next-generation disulfide stapling: reduction and functional re-bridging all in one.
Herein we present a significant step towards next-generation disulfide stapling reagents. A novel class of reagent has been designed to effect both disulfide reduction and functional re-bridging. The strategy has been applied to great success across various peptides and proteins. Moreover, application to a multi-disulfide system resulted in functional re-bridging without disulfide scrambling.
متن کاملNext-generation disulfide stapling: reduction and functional re-bridging all in one† †Electronic supplementary information (ESI) available: 1H and 13C NMR spectra for all small molecules, LC-MS, SDS-PAGE gels and UV-Vis analysis (where applicable) for all bioconjugates. See DOI: 10.1039/c5sc02666k Click here for additional data file.
Advances in protein modication by chemical means have led to the development of a range of protein bioconjugation methodologies. These methodologies have been successfully applied to a number of elds such as the uorescent tagging of proteins, the development of therapeutic protein conjugates to treat indications such as HIV, cancer and malaria, and for use as diagnostic tools. Whilst a large...
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ژورنال
عنوان ژورنال: Chemical Science
سال: 2016
ISSN: 2041-6520,2041-6539
DOI: 10.1039/c5sc02666k